Last edited by Dutilar
Tuesday, August 4, 2020 | History

3 edition of Towards a fibre optic nucleic acid biosensor found in the catalog.

Towards a fibre optic nucleic acid biosensor

Towards a fibre optic nucleic acid biosensor

thiazole organge derivatives as sensitive fluorescent probes to detect DNA hybridization.

  • 52 Want to read
  • 30 Currently reading

Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 1998.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL21871252M
ISBN 100612458482

Biosensors are valuable analytical devices that can be assimilated with on-line process monitoring schemes [].The basic principle of a biosensor is dependent on the affinity between the biological material and analyte (figure ) [].The selected biological material (usually a specific enzyme) is immobilized by conventional procedures (physical or membrane entrapment, non-covalent or covalent. selectivity and dynamic range of hybridization assays done using a fiber optic nucleic acid biosensor based on total internal reflection fluorescence (TIRF). Publication» Towards the development of a fiber-optic nucleic acid biosensor [microform]: an examination of .

  Please use one of the following formats to cite this article in your essay, paper or report: APA. Cuffari, Benedette. (, June 27). Detecting GM Foods with Fiber Optic Biosensors. Nucleic acids may be analyte or molecular recognition elements in biosensors. Both aspects merge in the genosensor approach, where detection of special sequences is facilitated by hybridization of a target nucleic acid to a complementary immobilized template.

Stanford Libraries' official online search tool for books, media, journals, databases, government documents and more. Nanobiosensors for Personalized and Onsite Biomedical Diagnosis [electronic resource] in SearchWorks catalog. Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. A recent development has been approved by the FDA.


Share this book
You might also like
Tom Browns schooldays.

Tom Browns schooldays.

Role and responsibility of teachers.

Role and responsibility of teachers.

The cool-kawaii

The cool-kawaii

Introduction to modern chemistry

Introduction to modern chemistry

Concrete crime.

Concrete crime.

The physical world of the Greeks

The physical world of the Greeks

Ecotales from Kubulau

Ecotales from Kubulau

New applications of membrane processes.

New applications of membrane processes.

powers, duties, and liabilities of town and parish officers in Massachusetts

powers, duties, and liabilities of town and parish officers in Massachusetts

World War II radio heroes

World War II radio heroes

New York States system of school finance, with examples from the 1975-76 school year

New York States system of school finance, with examples from the 1975-76 school year

handlist of British Parliamentary poll books

handlist of British Parliamentary poll books

Super baffler

Super baffler

Towards a fibre optic nucleic acid biosensor Download PDF EPUB FB2

A fibre-optic biosensor is described for detection of genomic target sequences from Escherichia coli.A small portion of the LacZ DNA sequence is the basis for selection of DNA probe molecules that are produced by automated nucleic acid synthesis on the surface of optical by: TOWARDS THE DEVELOPMENT OF A FIBRE-OPTIC NUCLEIC ACID BIOSENSOR: CONSIDERATIONS FOR THE QUANTITATIVE TRANSDUCTION OF HYBRIDIZATION OF IMMOBILIZED DNA James H.

Watterson The antibodies produced have selectivity towards binding a particular region of the antigen. This selective binding has been temed "molecular recognition"Author: James H. Watterson. Abstract. A fiber optic biosensor was used for the fluorimetric detection of T/AT triple-helical DNA formation.

The surfaces of two sets of fused silica optical fibers were functionalized with hexaethylene oxide linkers from which decaadenylic acid oligonucleotides were grown in the 3′ to 5′ and 5′ to 3′ direction, respectively, using a DNA by:   Fiber-optic biosensors (FOBS) are optical fiber-derived devices which use optical field to measure biological species such as cells, proteins, and DNA.

Because of their efficiency, accuracy, low cost, and convenience, FOBS are promising alternatives to traditional immunological methods for biomolecule by:   A Fiber-Optic Evanescent Wave DNA Biosensor Based on Novel Molecular Beacons. Analytical Chemistry71 (22), DOI: /acc.

Julia Cordek, Xinwen Wang, and, Weihong Tan. Direct Immobilization of Glutamate Dehydrogenase on Optical Fiber Probes for Ultrasensitive Glutamate by: The main components of FOBS (fiber optic biosensors) such as biosensing elements, fiber optic cables, optical element enhancements, transducers, sensing strategies, photodetectors, and signal processing are covered in detail by showcasing the recent developments in.

Optical fiber biosensors can be used in combination with different types of spectroscopic technique, e.g. absorp- tion, fluorescence, phosphorescence, Raman, surface plas.

Towards a fibre optic nucleic acid biosensor book developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR).

The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using.

applications of fiber-optic biosensors classified depending on the biorecognition element employed – enzymes, whole cells, antibodies, nucleic acids, and biomimetic polymers. Keywords Biosensor Optical sensors Fiber-optic Introduction Biological recognition elements have attracted extraordi.

Candiani A, Bertucci A, Giannetti S, et al. Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating. Nucleic acid functionalized evanescent wave fiber optic (EWFO) biosensors have attracted much attention due to their remarkable advantages in both device configuration and sensing performance.

One critical technique in EWFO biosensor fabrication is its surface modification, which requires (1) minimal nonspecific adsorption and (2) high-quality. DNA biosensors, based on nucleic acid recognition processes, are rapidly being developed towards the goal of rapid, simple and inexpensive testing of genetic and infectious diseases (2,3), and for the detection of DNA damage and interactions.

Unlike enzyme or antibodies, nucleic acid recognition layers can be readily synthesized and. A review of biosensor technology with a specific focus on fiber-optic biosensors is provided.

With the advent of optical transducer, better electronics, and improved immobilization methods, fiber. for a Fibre Optic DNA Biosensor Master of Science, L Stephen Chnstopher Jakeway Department of Chemistry University of Toronto Abstract This thesis reports studies of two fluorescent probes that could be used to detect DNA hybridization by a fibre optic nucleic acid biosensor that is being developed by our research group.

review on optical biosensors in general also covers fiber-optic biosensors17 andcontainssectionson(a)enzymaticbiosensors,(b) immunosensors, (c) biosensors based on ligand−receptor interactions, (d) nucleic acid biosensors, (e) whole cell biosensors, and (f) new materials for use in optical biosensors.

The first MOF-based biosensor has been implemented using a grapefruit geometry fiber, having five holes of μm diameter, forming an outer core of μm that includes a %wt Ge-doped socket of diameter μm, as shown in Figure In that fiber, a mm-long Bragg grating was inscribed using a nm phase mask and a nm excimer laser, 10 ns laser radiation (Pissadakis.

Both single optical fibers and optical fiber arrays have been used for biosensing based on biological recognition events. This chapter reviews progress and developments in the past decade on fiber-optic array platforms used for nucleic acid, enzyme, whole-cell, and immunoassay (antibody/antigen) biosensing.

DNA biosensors, based on nucleic acid recognition processes, are rapidly being developed towards the goal of rapid, DNA optical biosensors commonly rely on a fiber optic to transduce the emission signal of a fluorescent label.

Fiber optics are devices that carry light from one place to another by a series of internal inflections. Optical fiber-based biosensors combine the use of a biological recognition element with an optical fiber or optical fiber bundle. They are classified by the nature of the biological recognition element used for sensing: enzyme, antibody/antigen (immunoassay), nucleic acid, whole cell, and biomimetic, and may be used for a variety of analytes.

We have prepared a novel optical fiber evanescent wave DNA biosensor using a newly developed molecular beacon DNA probe. The molecular beacons (MB) are oligonucleotide probes that become fluorescent upon hybridization with target DNA/RNA molecules.

Biotinylated MBs have been designed and immobilized on an optical fiber core surface via biotin−avidin or biotin−streptavidin interactions. Moreover, these techniques have been utilized for the determination of numerous biological species as antigen, nucleic acids. Optical fiber biosensing is comprehensive and being commonly utilized for microorganisms identification Leung et al.

have described label free determination of DNA hybridization for pathogen testing by applying a fiber.The feasibility of a biosensor for DNA detection based on suspended-core photonic crystal fibers is investigated.

The possibility of functionalization of the hole surface, which allows DNA strand binding, is demonstrated, along with the selective detection of DNA through hybridization of immobilized peptide nucleic acid probes with their full-complementary and mismatched DNA segments.Fiber optic biosensors (FOBs) are increasingly being applied to the detection of bacteria in food and water supplies, food processing facilities, and homeland security operations.

These biosensors can be used for multiplexed pathogen detection or to confirm the results of other techniques, often in .